Team:KU Leuven/Journal
From 2013.igem.org
Secret garden
Congratulations! You've found our secret garden! Follow the instructions below and win a great prize at the World jamboree!
- A video shows that two of our team members are having great fun at our favourite company. Do you know the name of the second member that appears in the video?
- For one of our models we had to do very extensive computations. To prevent our own computers from overheating and to keep the temperature in our iGEM room at a normal level, we used a supercomputer. Which centre maintains this supercomputer? (Dutch abbreviation)
- We organised a symposium with a debate, some seminars and 2 iGEM project presentations. An iGEM team came all the way from the Netherlands to present their project. What is the name of their city?
Now put all of these in this URL:https://2013.igem.org/Team:KU_Leuven/(firstname)(abbreviation)(city), (loose the brackets and put everything in lowercase) and follow the very last instruction to get your special jamboree prize!
![tree](https://static.igem.org/mediawiki/2013/a/ac/Tree.png)
![ladybugcartoon](https://static.igem.org/mediawiki/2013/6/64/Ladybugcartoon.png)
Modelling
This summer, three dedicated male engineers spent a whole summer modelling our systems.
However, without the input from a girl, Tina, this would’ve been a rather nerdy page. Luckily, Tina was always there when the modelling team sought a balance between awful jokes and work efficiency. Nevertheless, without Sanders work tempo, Bert’s criticism and Tomas factotum mind, this team would not been as great as it was this year.
In this journal, you can read how they started modelling from scratch up to being modelling specialists, using Matlab, Symbiology, COBRA and even qPCR..
E-β-Farnesene
Here we present the work on the EBF-biobrick, a part of the glucose model, week by week. The contributors are Lukas and Su, which formed a very tight team that already started with lab work even during the exams in June. Thanks to Lukas’ explanations skills, several people were able to help them out in the lab when Lukas was busy multitasking. Su’s precise work and –for sure- his cute smile made sure everyone wanted to assist him in the lab this summer.
Nevertheless, Su and Lukas were our EBF guys from the summer! You can read all about the extensive work they have done in this part.
Feed forward loop
Long team discussions and Bert’s persistence resulted this summer in a ‘proof of concept’ wetlab version of Bert Mortier’s oscillator, called the ‘feed forward loop’.
Pieter and Sylvie were immediately up to form a subteam and started working in order to create a part of the oscillator.
In this part, you can read all about their big UP in the beginning, going to a down after a while and the eventual steady state with troubleshooting in the end.
Nevertheless, this feed forward loop is only the beginning to create Bert’s oscillator.
Methyl Salicylate
The Methyl Salicylate team or ‘MeS boys’, in normal life called Robber and Laurens, worked on the improvement of the methyl salicylate brick round the clock almost whole summer. The journal can be found here. More information on the MeS biobrick can be found on this page.
qPCR
We spent some time on performing a qPCR, a new and appeasing way to characterise and to find data for our computer model.