Team:TU Darmstadt/protocols/Heat Shock Transformation

Materials
Ice
Heatingbad
Pipets + steril tips
DYT-Media
LB-Agar-Plates + antibiotics
Incubator

Procedure
1. Defrost stocks of competent cells (100 µL in 1.5 ml Eppendorf tube) on ice
2. Add the DNA and incubate the suspension for 5 minutes on ice
3. Heat shock is done by incubating the cells for 1 minute at 42°C
4. Add 1 mL of DYT medium and incubate for 1 hour at 37°C in order to obtain antibiotic resistance
5. Finally the cells are ready to be crossed out