Team:TU Darmstadt/protocols/PCR

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PCR

Short report
 Materials
Equipment
 Micropipettes with sterile tips
PCR machine
PCR tubes

Chemicals & consumables
DNA tamplate
Nuclease-free water
Primer
Polymerase buffer
dNTP mix
DMSO

Procedure
 PCR program (30 cycles) initial denaturation 95°C, 60s denaturation 95°C, 45s annealing 54,5°C, 90s elongation 72°C, 120s final elongation 72°C, 120s run on an analytic 1% agarose gel purification using Wizard SV Gel and PCR Clean-Up System (Promega)

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