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From 2013.igem.org

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Sample Output

Automatically determined parameters using the [http://www.mediawiki.org/wiki/Table BioBrick-AutoAnnotator]

BioBrick: BBa_K1159000

Used open reading frame from position ?x? to ?y?.

A (Ala)

4 (10.53%)

R (Arg)

1 (2.63%)

N (Asn)

3 (7.89%)

D (Asp)

1 (2.63%)

C (Cys)

1 (2.63%)

Q (Gln)

2 (5.26%)

E (Glu)

4 (10.53%)

G (Gly)

1 (2.63%)

H (His)

2 (5.26%)

I (Ile)

1 (2.63%)

L (Leu)

3 (7.89%)

K (Lys):

2 (5.26%)

M (Met)

2 (5.26%)

F (Phe)

1 (2.63%)

P (Pro)

2 (5.26%)

S (Ser)

3 (7.89%)

T (Thr)

1 (2.63%)

W (Trp)

2 (5.26%)

Y (Tyr)

1 (2.63%)

V (Val)

1 (2.63%)

Amino acid counting:

Total number of amino acids (aa):

38

Number of positively charged aa (Arg + Lys):

3

Number of negatively charged aa (Asp + Glu):

5

Biochemical parameters:

Molecular weight [Da]:

4432.97

Theoretical pI:

5.42

Extinction coefficient:

?4?

Estimated half-life:

Mammals:

?4 h?

Yeast:

?4?

E. coli:

?4?

Codon usage:

Mammals:

?good?

Yeast:

?Ok?

E. coli:

?bad ?

RFC standard:

This is a RFC 25 BioBrick, thus ATGGCCGGC and ACCGGT were added to the 5' and 3' ends.

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