Team:KU Leuven/Project/Modelling/Cellular Level

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Secret garden

Congratulations! You've found our secret garden! Follow the instructions below and win a great prize at the World jamboree!


  • A video shows that two of our team members are having great fun at our favourite company. Do you know the name of the second member that appears in the video?
  • For one of our models we had to do very extensive computations. To prevent our own computers from overheating and to keep the temperature in our iGEM room at a normal level, we used a supercomputer. Which centre maintains this supercomputer? (Dutch abbreviation)
  • We organised a symposium with a debate, some seminars and 2 iGEM project presentations. An iGEM team came all the way from the Netherlands to present their project. What is the name of their city?

Now put all of these in this URL:https://2013.igem.org/Team:KU_Leuven/(firstname)(abbreviation)(city), (loose the brackets and put everything in lowercase) and follow the very last instruction to get your special jamboree prize!

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Synthetic biology designs new networks within/ on top of the existing cellular network of your favourite organism.The key question, however, is whether the cell/tissue/organism will actually be able to produce this network?! Hence, several questions arise on our way to this goal: - Are there sufficient tRNA’s to build these new proteins, or should we codon optimise our strains? - Will the cell be able funnel off enough precursor material to build significant amounts of this new network? - Will the series of proteins and enzymes produce our final, desired product? - Won’t the cell make toxic side-products in the process, possibly reducing growth and thus production rate?

Methyl Salicylate - Modelling: Flux Balance Analysis

We ran the Flux Balancing Analysis using the COBRA Toolbox for MATLAB. With this analysis we checked whether the growth rate of our cells will be affected when we introduce our methyl salicylate system.

Methyl Salicylate - Modelling: Kinetic Parameters Model

We have modelled the production system of methyl salicylate by using the transcription, translation and protein degradation rates in order to calculate the mRNA and protein fluxes. We also brought the kinetics of methyl salicylate synthesis into account.

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