Team:Groningen/Labwork/25 June 2013
From 2013.igem.org
Mirjam, Chaline and Inne
A couple of media are prepared:
LB medium with agar 1 liter
LB medium divided into 80 ml portions
70% ethanol
2 liter TBE buffer Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving the LB medium with agar is placed in the 55 degrees Celsius stove.
Mirjam and Claudio
''B. subtilis'' strain 168 is inoculated O/N at 37 degrees Celsius and every buffer necessary for the extraction of genomic DNA is arranged.
Claudio
The design for the heat motility sub-project continues.