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Team:Goettingen/NoteBook w1
From 2013.igem.org
June 5th, Wednesday
We prepared the medium, antibioticks and started the labwork from transformation.
Preparation of Antibiotic Stocks- 1000x Ampicillin 10 1mL Stocks (EPs in the red box in -20 freezer)
- 1000x Chloramphenicol 10mL Stock (Falcon in Freezer)
- 250ml*4 LB media with Cm
- 250ml*1 LB media with Amp
| Code Name | Description |
|---|---|
| iGEM_36 | DarR operator sequence + biobrick prefix |
| iGEM_37 | DarR operator sequence + biobrick sufix |
Transformation
| Biobrick entry number | Mark |
|---|---|
| BBa_J23117 | part 1 |
| BBa_J23116 | part 2 |
| BBa_J23110 | part 3 |
| BBa_J23118 | part 4 |
| BBa_J61101 | part 5 |
| BBa_BE0240-Cm | part 6 -- Cm |
| BBa_B0015 | part 7 -- Cm |
| BBa_B0034 | part 8 |
| Resuspended but not transformed | |
| Entry number | location on kit |
| BBa_E0204-Amp | P5 12 M |
| BBa_QO3121 | P5 20 N |
June 4th, Tuesday
We find the correct DNA sequence of DarR and designed primers for it
| Code Name | Description |
|---|---|
| iGEM_32 | Forward primer for DarR ORF amplification, with biobrick prefix |
| iGEM_33 | Reverse primer for DarR ORF amplification, with biobrick sufix |
| iGEM_34 | Forward primer for DarR ORF PCR amplification sequencing. |
| iGEM_35 | Reverse primer for DarR ORF PCR amplification sequencing. |
