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From 2013.igem.org

Nanodropping overnight incubations of herring sperm DNA

• Table representing the concentrations of herring sperm before and after different treatments

Sample	Concentration
Sheared/Unsonicated (s.u.s)	17900ng/ul=17.9mg/ml
S.u.s 10fold dil.	1790.1ng/ul
S.u.s 100fold dil	152.6ng/ul
Sheared/Sonicated (s.s)	17667ng/ul=17.6mg/ml
S.s 10fold dil.	1766.7ng/ul
S.s 100fold dil.	156.2ng/ul
Sheared/sonicated overnight	Concentration in the tube=Average of the two samples=16.9mg/ml
500ul(DNA) in 500ul(1xTE)	14485.9ng/ul
2ml(DNA) in 10ml(1xTE)	3883ng/ul

• Concentrations decrease with dilutions
• The stock concentration in sheared/sonicated tube varies slightly, when derived from measured dilution concentrations that were subjected to different treatments (i.e. overnight incubation), though they orignated from the same tube
• Difference in concentration is not too significant though: proposed 17.6mg/ml vs 16.9mg/ml