"
User contributions
From 2013.igem.org
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
- 03:19, 27 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Project
- 03:17, 27 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Project (→results)
- 03:16, 27 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Project
- 03:15, 27 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Project (→results)
- 03:13, 27 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Project (→results)
- 02:57, 27 September 2013 (diff | hist) N Index. check the function of the xylose and the nitrate inducible promote (Created page with "<p>1>Prepare the 10% xylose solution and the 1mol/l nitrate solution, then filter.</p> <p>2>Transform the biobrick into the competent cells, and culture them for 1h.</p> <p>3>Pre...") (top)
- 02:54, 27 September 2013 (diff | hist) Index.The recycle of the products of the PCR (top)
- 02:54, 27 September 2013 (diff | hist) Index.The recycle of the products of the PCR
- 02:51, 27 September 2013 (diff | hist) N Index.The recycle of the products of the PCR (Created page with " The recycle of the products of the PCR 1> Transfer the products of the PCR into a new 1.5ml centrifuge tube, then estimate the amount of the liquid, then add 3 times the volum...")
- 02:49, 27 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Notebook
- 02:49, 27 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Notebook
- 02:48, 27 September 2013 (diff | hist) N Index. recycle of the DNA in the gel and deal with gel (Created page with "<p>1>After collect the gel, weight it and crush it with 1ml tip or centrifuge tube.</p> <p>2>Add the same volume of the solution1, then vortex or put it upside down.</p> <p>3>Hea...") (top)
- 02:42, 27 September 2013 (diff | hist) N Index.Preparation of the gel (Created page with "<p>1>Weigh agarose in 1% mass ratio of the whole solution.<br> 2>Put the agarose in one-time TAE buffer.<br> 3>Use microwave oven to dissolve the agarose totally.<br> 4>Wait the ...") (top)
- 02:40, 27 September 2013 (diff | hist) N Index. Usage of FCM (Created page with "<p>1>Firstly open the instrument and the computer of it.<br> 2>Secondly use ddH2O to wash the access inside the FCM,<br> 3>Then have our bacteria solution diluted for 20 times in...") (top)
- 02:39, 27 September 2013 (diff | hist) N Index.Preparation of the nitrate LB culture (Created page with "<p>1>Calculate the nitrate of potash in the needing concentration of the given volume.<br> 2>Weigh the nitrate of potash as we have calculated.<br> 3>Dissolve the nitrate of poas...") (top)
- 02:38, 27 September 2013 (diff | hist) N Index. Preparation for the M9 culture (Created page with "<p>1> Firstly prepare for the five-time M9 salty solution.<br> M9 solution recipe (1L):<br> 64g Na2HPO4, 15g KH2PO4, 2.5g NaCl, 5.0g NH4Cl. Add the ddH2O to dissolve all the solu...") (top)
- 02:36, 27 September 2013 (diff | hist) N Index.Usage of ultraviolet spectrophotometer (Created page with "<p>1>Firstly detect the density of the culture in the cuvette, and set the tare button. <br>2>Inject the relevant bacteria solution into the cuvette, let the UV go through it. <b...") (top)
- 02:35, 27 September 2013 (diff | hist) N Index.Usage of PCR-Cycler (Created page with "<p>1> Determine the annealing temperature according to the number -5 behind the TM(0.5M Na+) in the primer usage instructions. In order to prepare the concentrated solution, dete...") (top)
- 02:33, 27 September 2013 (diff | hist) N Index.The use of spectrometer (Created page with "<p> 1>Absorb pluxRI2 CM+ from the solution, next, dilute it into 30ml with five kinds of concentrations, 1:10, 1:20, 1:30, 1:40, and 1:50, then inject 22ul CM into it.</p> <p>2> ...") (top)
- 02:32, 27 September 2013 (diff | hist) Index.Extract the target DNA (top)
- 02:31, 27 September 2013 (diff | hist) N Index.Extract the target DNA (Created page with "<p>Extract the target DNA.<br> Materials: bacteria(in the liquid culture), reagent case.<br> <br>Methods:</p> <p>1. After centrifuging the bacteria, collect them in the same EP ...")
- 18:18, 26 September 2013 (diff | hist) N Index.Preservation of the bacteria (Created page with "<p>1>Bacteria must be kept in the -80’c circumstance dissolved in the glycerol, and the content of the glycerol is roughly 20% to 30%.<br> Materials: 40% glycerol, ba...") (top)
- 18:17, 26 September 2013 (diff | hist) Index.Transformation (top)
- 18:15, 26 September 2013 (diff | hist) N Index.Transformation (Created page with "<p>1.Preparation for the competent cells in the -80’c refrigerator, and target DNA molecular. Then refresh the competent cells in the 0℃ icy water. <br> Mix the two component...")
- 18:14, 26 September 2013 (diff | hist) N Index.Preparation of some antibiotic solution (Created page with "<p>1.Cam antibiotic is water-soluble , 10mg/ml, preserved in the -20’c environment.<br> 2. Kam antibiotic must be dissolved in the alcohol, 34mg/ml, preserved in the -20’c en...") (top)
- 18:12, 26 September 2013 (diff | hist) Index.Preparation of LB culture (top)
- 18:12, 26 September 2013 (diff | hist) N Index.Preparation of LB culture (Created page with " <p>1>LB solid recipe(1L):<br> yeast extract 5g<br> agarose(100ml 1.5g) <br> sodium chloride 10g <br> peptone 10g. <>br and the liquid recipe, based on the mater...")
- 18:09, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Notebook
- 18:05, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Notebook
- 18:01, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Notebook
- 17:58, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Notebook
- 17:57, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Notebook
- 17:55, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Notebook
- 17:55, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Notebook
- 17:54, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Notebook
- 17:44, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Project (→project results)
- 17:41, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Project (→project results)
- 17:34, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Project
- 17:17, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Project
- 17:06, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Project
- 16:57, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Project
- 16:47, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Modeling
- 16:46, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Modeling
- 16:45, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Modeling
- 16:37, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A
- 16:36, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Project
- 16:35, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Safety
- 16:35, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Attributions
- 16:34, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Attributions
- 16:33, 26 September 2013 (diff | hist) Team:SUSTC-Shenzhen-A/Human Practice
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
