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Exeter/3 September 2013
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Revision as of 14:43, 3 September 2013 by JoshMiddleton (Talk | contribs)
Contents |
Results of liquid cultures from yesterday
gBlocks
| gBlock | Colour |
|---|---|
| 1 | No |
| 2 | No |
| 3 | Vibrant pink |
| 4 | No |
| 5 | Vibrant pink |
| 6 | No |
| 7 | Peach |
| 8 | No |
| 9 | Pink - not so vibrant |
| 10 | Vibrant pink |
| 11 | Vibrant pink |
| 12 | Pink - not so vibrant |
| 13 | Pink - not so vibrant |
| 14 | No |
| 15 | No |
| Part | Did it work? |
|---|---|
| OmpF + RBS A | ✓ |
| OmpF + RBS B | ✓ |
| OmpF + RBS C | ✓ |
| OmpF + RBS D | ✓ |
| K592022 A | ✗ |
| K592022 B | ✓ |
| K592011 A | ✗ |
| K592011 B | ✓ |
| K864404 | ✓ |
Miniprepped
- OmpF + RBS A
- OmpF + RBS B
- OmpF + RBS C
- OmpF + RBS D
- K592011 B
- gblocks 1 to 15
NanoDrop from miniprep
| Part | Concentration (ng/ul) |
|---|---|
| CcaR | 324.6 |
| CcaS | 264.4 |
| Yellow | 64.3 |
| Magenta | 102.7 |
| RBS | 48.9 |
| Promoter, RBS, #1 | 21.5 |
| Promoter, RBS, #2 | 22.6 |
| Promoter, RBS, #3 | 22.0 |
| Fix L | 31.9 |
| Fix J | 35.4 |
| YF1 | 27.1 |
| OmpF | 28.2 |
