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From 2013.igem.org

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• iGEM 2013

Cholera Detection May - June Notebook: July 1 - July 7 Daily Log

Overview March-April May-June July-August September-October

9/4/2013 9/6/13 Our results from the side-by-side plate comparison of grown cholera with several strains of bacteria are encouraging! We grew V. cholerae from one week Clarice transformed the iGEM backbone into E.Coli and grew it up in an overnight. From those cells we isolated the plasmid, pIG91, in two Eppendorfs, at concentrations of 52 ng/uL and 82 ng/uL. Monday we will digest the plasmid and the SdiA gene to splice it in and submit to the registry.