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Team:BYU Provo/Notebook/CholeraDetection/Springexp/Period1/Dailylog
From 2013.igem.org
| Cholera Detection May - June Notebook: July 1 - July 7 Daily Log
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9/4/2013
9/6/13 Our results from the side-by-side plate comparison of cholera with several strains of bacteria are encouraging! We grew a patch of V. cholerae for one week on LB plates. Then, we streaked a line of a bacteria adjacent to the patch and another away from the patch. We did this for six different strains on six different plates. None of the bacterial strains plated adjacent to cholera grew. All strains grew away from cholera. However, TT9907, the strain with lambda lysogen, showed plaques! There were distinct plaques on the line of bacteria leading away from Cholera. Through what we believe is a quorum sensing pathway, the lambda lysogen recognizes that E.Coli has detected cholera. It mobilizes excision from the genome, replicates, and lyses it's host. We prepared top agar lawns of our 6 strains, with cholera plated in the middle, to show more clearly the result we saw today. Clarice transformed the iGEM backbone into E.Coli and grew it up in an overnight. From those cells we isolated the plasmid, pIG91, in two Eppendorfs, at concentrations of 52 ng/uL and 82 ng/uL. Monday we will digest the plasmid and the SdiA gene to splice it in and submit to the registry.
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