Team:BYU Provo/Notebook/SmallPhage/Winterexp/Period4/Dailylog

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Small Phage March - April Notebook: April 1 - April 14 Daily Log

4/17/13

- Gave our presentation in class on how we would select for small phage (ADD PRESENTATION LINK)

- Discussed plans for finals week and interim period

4/24/13

- Started 5mL of E. coli BL21 overnight liquid culture at 11:15pm from our 4.6 BL21 streak.

4/25/13

- Checked up on E. coli liquid culture and performed streaking.

- Performed T7 Phage Viability Test (ADD LINK)

- Started overnight E. coli liquid culture using 4.6 streak

4/26/13

- Checked up on results from T7 Phage Viability Test (ADD LINK)

- Found that the streak from 4.25 did not work very well since there were no individual colonies.

- Performed 4.26 T7 Phage Selection Test (ADD LINK)

- Started a new streak using the 4.25 overnight

- Started 3mL overnight of E. coli from 4.6 streak

4/27/13

- Checked up on 4.26 T7 Phage Selection Test (ADD LINK)

- Started phage liquid culture

+ phage: 4mL LB + 1mL E coli + 100μL phage stock

– phage: 4mL LB + 1mL E coli