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From 2013.igem.org

{ "date" : "2013-06-22", "author" : "bruno", "title" : "Minipreps and screening of Promoters", "content" : "From inocula of the day before I made the minipreps by the followingprotocol.

I made the screening test. To do that we digested 1500 ng of DNA with EcorI and PstI following the screening digestion protocol.
I used a 1.5% concentration of agarose to create a gel and I used Ethidium bromide instead the normal EuroSafe. In addition I have not used a normal Dye with the 20ul of sample loaded but I used 4ul of 30% glycerol. ", "tags" : "lacOid-PliaG-Pgram+" }