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From 2013.igem.org

Project Description

pH based bacterial systems, wherein response is dependent on the pH of the medium have been explored rarely in iGEM (NYMU-Taipei(2008), Wisconsin-Madison(2010) others). pH based sensor systems have potential use in bio-remediation, gut-microbiota engineering, fermentation et cetera.

pH based systems present various other challenges with respect to biobrick usability, pH dependence of reporters and lack of characterization.Our aim is to create a simple pH "bacterial" meter, not unlike a pH strip used in chemistry. We hope for this to be a template for future projects and applications

Abstract

pH induced response elicited by certain promoters in bacteria may have major practical applications. The response can be targeted for specific pH ranges, for example in tracking the anomalies associated with the gut micro-biota or detecting pH inside a bioreactor. There are only limited studies reported in the area. In the present project, a genetic circuit has been created, using the promoters of the acid shock response gene from E.coli and the F0F1 ATPase operon from C. glutamicum that produces a pH dependent colour gradient, much like a universal pH indicator. A mathematical model has been developed to simulate the experimental findings. The present study will form the basis for further research in the field of synthetic biology.

Contact

You can send us queries at igemiitdelhi2013 at gmail dot com . Keep watching this space for more from the team.