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12/09/13
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Revision as of 09:33, 13 September 2013 by AliceHaworth (Talk | contribs)
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Isolate plasmid from overnight culture
- Using Omega mini prep kit protocol
- Samples:
- TODF 1,2,3,
- TODX 1, 2, 3
- TOBG 1, 2, 3,
- RFP 1, 2, 3
| sample | Volume (ul) | Concentration (ng/ul) |
| TODF1 | 43 | 120 |
| TODF2 | 43 | 98.8 |
| TODX1 | 42 | 399.3 |
| TODX2 | 44 | 442.2 |
| TODX3 | 43 | 417.5 |
| TOBG1 | 43 | 262.4 |
| TOBG2 | 47 | 319.2 |
| TOBG3 | 41 | 338.4 |
| RFP1 | 41 | 77.7 |
| RFP2 | 41 | 32.7 |
| RFP3 | 42 | 59 |
Glycerol stocks
- Took 750ul from overnight culture and centrifuged at full speed for ~2 minutes
- Supernatant was removed
- Pellet resuspended in 375ul of HMFM
- Samples were then frozen at -80
Double Digest
