Team:BYU Provo/Notebook/Phage Purification/Springexp/Period3/Dailylog

Overview

March-April

May-June

July-August

September-October

5.26 PEG Purification

5/31/13

- Worked on setting up our notebook online.

6/3/13

- Ran the CsCl purification step.

6.3 CsCl Gradient Phage Purification

- Next class we will attempt extracting the phage from the gradient.

6/5/13

- Extracted the phage from 6.3 CsCl Gradient Phage Purification using a syringe and a needle.

Only extracted T4 because the T7 gradient was destroyed in the refrigerator.

Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed.

Placed these in the refrigerator.

- Prepared stocks of bacteria for future use.

6.5 Bacteria Cultures

6/7/13

Made 1 L of 10 X phage suspension buffer -added12.4 g Tris HCl to 1 L H2O and titrated to 7.20 pH using NaOH (50%) (.1 M) -added 58.44 g NaCl (1 M) -added 9.52 g MgCl2 (.1 M)

Autoclaved and stored for use in dialysis after CsCl gradient.

3/22/13

3/25/13

3/27/13

3/29/13