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Team:NCTU Formosa/safety
From 2013.igem.org
Inside a newly built, well-equipped laboratory, we conducted our experiment. Besides safety equipment, each member is well informed of the safety rules and emergency handling.
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SAFETY OF OUR PROJECT
Our project is to create a regulation system of gene expression in bacteria, only create new biobrick without producing any biosynthesis product. So it won't have new risks if we used our part in commerce or industry.We had discussed with National Chiao Tung University Environmental Protection and Safety Center.The committee agree us executing our project without any comment.
ORGANISMS WE USED
| Species | Strain Number | Risk group | Disease risk to human |
|---|---|---|---|
| E.coli | DH5α | 1 | May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. |
NEW OR MODIFIED CODING REGION WE USED
| Part number | Where to get this part | Originally come from | Risk group | Function |
|---|---|---|---|---|
| BBa_K1017301 | addgene | Synechocystis | 1 | chimeric light receptor function of the photoreceptor Cph1 |
| BBa_K1017301 | genedireX | E. coli | 1 | egulating gene expression |
| BBa_K1017302 | genedireX | E. coli | 1 | egulating gene expression |
SAFETY PROCEDURE IN OUR LABORATORY
Instruments and equipment in Our Laboratory
Figure.1 Biosafety cabinet
Figure.2 4℃ Refrigerator
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Figure.3 Sterilizer
Researcher's Safety
In order to protect researcher from infecting engineered organisms or contaminating the samples, we use the following procedures: 1.Researchers who are doing experiment must wear on lab coat and gloves. Figure.5 Researcher's wearing(待補) 2.No experiments is allowed to be done outside the biosafety cabinet.
Pubilc Safety
To ensure that the engineered organisms will not leak out to the environment, all wastes which contacted these bacteria should be sterilized before disposal.
Figure.3 Sterilizer
