Team:BYU Provo/Notebook/CholeraDetection/Springexp/Period2/Dailylog

From 2013.igem.org

Revision as of 22:29, 16 September 2013 by Kiserk (Talk | contribs)


Cholera Detection September-October Notebook: September 16 - September 29 Daily Log



Overview
March-April
May-June
July-August
September-October


9/16/13

On Saturday we checked our bacteriophage spot test for plaques, but we saw none. Our bacteriophage lambda purification procedure failed. Dr. Grose thinks that inducing lambda to lysis by 43 degree heat shock must not have been sufficient stress. Since we know H202 does induce our phage, we're going to attempt to isolate bacteriophage lambda by doing a top agar plaque assay with a drop of hydrogen peroxide, then bathing the top agar in LB, sucking it all up and then purifying the bacteriophage by chloroform lysis procedure.

Clarice has been working on submitting parts to the registry. She has cloned the Ydiv gene into the iGEM backbone to submit the part; we purified the plasmid for submission.


9/18/13


Retrieved from "http://2013.igem.org/Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period2/Dailylog"