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Team:BYU Provo/Safety
From 2013.igem.org
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Organism Used<to be deleted>Use this page to answer the questions on the safety page. Chassis Organisms
Highest Risk Group ListedGreater than 1. For more information, please refer to
Coding Region Used
RisksDo the biological materials used in your lab work pose risks to the safety and health of team members or others working in the lab?Yes. Vibrio cholerae can pose a risk to team member safety if ingested, handled improperly, or improperly disposed of (causing accidental exposure). Do the biological materials used in your lab work pose risks to the safety and health of the general public, if released by design or by accident?There are no plans for release of anything harmful. The safety practices, including proper disposal, were designed after consultation with a V. cholerae and bacterial pathogen expert, [http://lifesciences.byu.edu/~rar5 Dr. Richard Robison], BYU. Do the biological materials used in your lab work pose risks to the environment, if released by design or by accident?There are no risks associated with the environment. Do the biological materials used in your lab work pose risks to security through malicious misuse by individuals, groups, or countries?We believe our safety practices in the lab prevent malicious misuse by individuals, groups, and countries. If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, what new risks might arise? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?There would be no known risks. Our chassis organism is a BSL 1 E. coli. Of course caution must be used in any case. The bacteriophages we used also must be tested before any human use.
Safety PrecautionsDoes your project include any design features to address safety risks?Not yet. What safety training have you received (or plan to receive in the future)?All members of the team have received safety training from our instructors as well as an [http://training.byu.edu/ytrain online safety training] run by our university, BYU. Please provide a link to your institution biosafety guidelines.[http://lifesciences.byu.edu/safety/LabSafety/SafetyPolicies.aspx College of Life Sciences - Safety Policies] Does your institution have an Institutional Biosafety Committee, or an equivalent group? If yes, have you discussed your project with them? Describe any concerns they raised with your project, and any changes you made to your project plan based on their review.We operate under the rules and guidelines as outlined on the [http://lifesciences.byu.edu/safety/Home.aspx BYU College of Life Sciences' Safety Page]. An IBC application must be filed for recombinant DNA. We have filed out this IBC report and our project has been approved the BYU Research Safety committee. Does your country have national biosafety regulations or guidelines? If so, please provide a link to these regulations or guidelines if possible.[http://www.cdc.gov/biosafety/publications/bmbl5/index.htm Biosafety in Microbiological and Biomedical Laboratories (BMBL) 5th Edition] According to the WHO Biosafety Manual, what is the BioSafety Level rating of your lab?BioSafety 2 What is the Risk Group of your chassis organism(s), as you stated in question 1? If it does not match the BSL rating of your laboratory, please explain what additional safety measures you are taking.BioSafety 1
Biosafety Form Part 2
Form Download
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