17/09/13

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Results from previous day

  • Background control plate had growth, which was not expected. This suggests that pSB1C3 backbone religated.
  • It was also thought that the Tod genes used were not from the PCR fusion experiment.
  • Same experiment done again, with a new set of Tod genes from a PCR fusion.
  • For digestion of backbone, already digested backbone from 01/08/2013 was used, which was digested with EcoRI and PstI.

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