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Team:XMU-China/Safety1
From 2013.igem.org
Project Safety
To make sure that our project is safe, we have taken the laboratory safety, public safety and environmental safety into account. Any safe risks or hazards would be found out and get rid of.
Biosafety Standards and Institutions
Xiamen University has its own biosafety rules that are regulated by the Ministry of Environment Protection, P.R. China and our institution. We carried out our experiments and evaluated bio-safety issues in our iGEM project strictly according to the Chinese Pathogenic Microbiology Laboratory Bio-safety Regulations, 2004, P.R. China.
Environmental Safety
We only used nonpathogenic Escherichia. Coli(DH5α and BL21(wild type)) to be host cell, which is a biosafety level 1 organism in our project. However, Escherichia. Coli (DH5α and BL21) can survive departing from the lab. Inasmuch as , we disinfect the discard raffinate strictly to deal with it.
Biosafety Training
Every member of our laboratory received biosafety training before the project began. It included general laboratory safety, biosafety and hazardous and chemical spill training. These training sessions were put on by the code of practice and the local guidelines, and including the safety and operations manual as well. Furthermore, every member of the iGEM team was trained by our iGEM graduate student instructors on proper laboratory techniques and safety procedures.
1. List and describe all new or modified coding regions you will be using in your project. (If you use parts from the 2013 iGEM Distribution without modifying them, you do not need to list those parts.)
| Part number. | Where did you get the physical DNA for this part (which lab, synthesis company, etc) | What species does this part originally come from? | What is the Risk Group of the species? | What is the function of this part,in its parent species ? | |
| Ex | BBa_C0040 | Synthesized, Blue Heron | Acinetobacter baumannii | 2 | Confers tetracycline resistance |
| 1 | BBa_K1036001 | PCR, Dr. I-Son Ng' s lab, Xiamen University |
Escherichia coli BL21 (DE3) | 1 | coding for respiratory NADH dehydrogenase 2/cupric reductase |
2. Please describe the chassis organism(s) you will be using for this project. If you will be using more than one chassis organism, provide information on each of them:
| Species | Strain no/name | Risk Group | Risk group source link | Disease risk to humans? If so, which disease? | |
| Ex | E. coli (K 12) | NEB 10 Beta | 1 | www.absa.org/riskgroups/bacteria search.php?genus=&species=coli | Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. |
| 1 | E. coli (K 12) | DH5α | 1 | http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia | Yes.May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. |
| 1 | E. coli (K 12) | DH5α | 1 | http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia | Yes.May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. |
| 2 | E. coli (B) | BL21 (DE3) | 1 | http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia | Yes.May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. |
| 3 | E. coli (K 12) | MG1655 | 1 | http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia | Yes.May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. |
| 4 | BL21 | DH5α | 1 | http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia | Yes.May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. |
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