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Team:NCTU Formosa
From 2013.igem.org
We have proven a sRNA-regulated system of our own to be an effective and competent way for regulating gene expressions.
Recent studies have shown that sRNA-mediated regulation is an important factor to bacterial growth. sRNAs work by base pairing with limited or extended complementary target mRNAs, regulating protein productions. Using sRNA mechanism, we can control gene expression in RNA level, in contrast to common promoters that functions on DNA level. Since the existing sRNAs in Escherishia Coli have important functions in other metabolic processes, we designed an artificial sRNA with high specificity to avoid undesired base binding in vitro.
By using the sRNA-regulated system, red light induced ooperator, and thirty seven degree Celsius ribosome binding site (RBS), we constructed a manipulatable system that is capable of expressing four different genes under different conditions. In other words, it is a multitask machine.
E.colightuner
Using sRNA, temperature and light, we want to construct a complicated regulation system. First, we took advantage of a powerful regulated molecules, sRNA. Bacterial small RNAs(sRNA) are small non-coding molecules, and they are highly structured and contain several stem-loops. sRNAs can either bind to protein targets, and modify the function of the bound protein , or bind to mRNA targets and regulate gene expression. Second, we used a Ribosome binding site which can sense change of temperature, 37oC RBS. Because this sequence has a unique secondary structure, it can’t function in room temperature. When temperature increasing, the secondary structure would change and can function. Third, we designed a new promoter, Pred. It is activated by red light. When removing the red light, it would be turned off, so we use this promoter to strengthen our project. By these important factors, we can achieve the pathway regulation and do more applications.
