Notebook

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Week 1 - July 8^th-14^th

Culturing of magnetotactic bacteria (MTBs)
Making base media for growing magnetotactic bacteria. This media was also used to make agar plates. MS-1 inoculated in liquid base medium. Falcon tubes were flushed with the nitrogen before use and incubated at 28 degrees. Plates were kept in a closed container with an Oxoid AnaeroGen pad.

Assembly of constructs
Glycerol stock of E. coli with pBBR1MCS-2 was thawed to grow on these plates. Primers for MamC (MS-1 strain) was ordered.

Team photo in the park at Frederiksberg Campus

Week 2

Assembly of constructs Overnight liquid cultures of pBBR1MCS-2 and eGFP plasmid (Adam Takos)

Touchdown PCR amplification of MamC from MS-1 gDNA (both Phusion and Hotmaster) and of eGFP (only Hotmaster).

This week the team...

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Team:UNIK Copenhagen/Signe Notebook

From 2013.igem.org

Notebook

Week 1 - July 8th-14th

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7

Week 8

Week 9

Week 10

Week 11

Week 12

Week 1 - July 8^th-14^th