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Template:Kyoto/Notebook/Aug 10
From 2013.igem.org
Aug 10
Ligation
| state | Vector | Inserter | Ligation High ver.2 | ||
|---|---|---|---|---|---|
| experiment | Pconst | 13.2 | spiinach-DT | 2.1L | 3.5 |
| experiment | Plac | 15.3 | spiinach-DT | 2.1 | 3.5 |
| experiment | Pconst | 13.2 | pT181 antisense (XbaI & PstI) | 6.6 | 3.5 |
| experiment | Plac | 15.3 | pT181 antisense (XbaI & PstI) | 6.6 | 3.5 |
| experiment | Ptet | 30.1 | pT181 antisense (XbaI & PstI) | 6.6 | 3.5 |
| experiment | Pconst | 13.2 | pT181 attenuator | 11.7 | 3.5 |
| experiment | Plac | 15.3 | pT181 attenuator | 11.7 | 3.5 |
| experiment | Ptet | 30.1 | RBS-lacZα-DT | 3.0 | 3.5 |
incubate 16°C 1hour
