Template:Kyoto/Notebook/Aug 10

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Aug 10

Ligation

Kojima

stateVectorInserterLigation High ver.2
experimentPconst13.2spiinach-DT2.13.5
experimentPlac15.3spiinach-DT2.13.5
experimentPconst13.2pT181 antisense (XbaI & PstI)6.63.5
experimentPlac15.3pT181 antisense (XbaI & PstI)6.63.5
experimentPtet30.1pT181 antisense (XbaI & PstI)6.63.5
experimentPconst13.2pT181 attenuator11.73.5
experimentPlac15.3pT181 attenuator11.73.5
experimentPtet30.1RBS-lacZα-DT3.03.5

incubate 16°C 1hour