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Phage Purification March - April Notebook: Experiments

Overview March-April May-June July-August September-October

4.2 W3110 Liquid Culture I) Purpose Grow bacteria for use in experiments. II) Expected Outcome Viable liquid culture of E. coli, W3110 III) Reagants Used W3110 plated colonies LB IV) Actual Procedure We put a colony from our W3110 plate of E.coli into 1mL LB broth and left in shaker at 37◦ overnight. Repeated 4 times, leaving a total of 5 1 ml colonies. V) Results Growth of W3110 liquid cultures of bacteria to create glycerol stocks from. The control tube showed no contamination.