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Template:Kyoto/Notebook/Aug 11
From 2013.igem.org
Aug 11
Miniprep
| DNA | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| 8/9 BBa-J44000-(2) | 100 | ||
| 8/9 BBa-J23106 | 154 | ||
| 8/9 BBa-J23100 | 144 | ||
| 8/10 T7-His-FT | 92 |
Colony PCR
| Sample | base pair |
|---|---|
| BBa-B0034-(2) | - |
| BBa-B0034-(1) | - |
| BBa-R0011-(2) | - |
| BBa-R0011-(1) | - |
| negative control | - |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94°C | 94°C | 55°C | 68°C | -- |
| 5min | 30s | 30s | 20s | 30 |
| Sample | base pair |
|---|---|
| BBa-D0412-(1) | |
| BBa-D0412-(2) | |
| negative control |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94°C | 94°C | 55°C | 68°C | -- |
| 5min | 30s | 30s | 1min30s | 30 |
