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Template:Kyoto/Notebook/Aug 10
From 2013.igem.org
Contents |
Aug 8
Aug 10
Liquid Culture
| Sample | medium |
|---|---|
| 8/9 BBa-J23106 | LB(+amp) |
| 8/9 BBa-J23100-1 | LB(+amp) |
| 8/9 BBa-J23100-2 | LB(+amp) |
| 8/9 BBa-J44000-1 | LB(+amp) |
| 8/9 BBa-J44000-2 | LB(+amp) |
Waking Up Parts
| Sample |
|---|
| BBa-Q04400(RBS-tetR-DT-Ptet)pSB2K3 |
| BBa-B0034(RBS)pSB1A2 |
| BBa-I732020(RBS-lacZα-DTt)pSB1C3 |
| BBa-P0412(RBS-lacI-DT)pSB1C3 |
| BBa-R0011(Plac)pSB1C3 |
LB Medium Plate(+Ampicillin)
| volume | 200ml |
|---|---|
| Bacto T2ypton | 2g |
| Bacto yeast extract | 1g |
| NaCl | 1g |
| 0.4M NaOHaq | 500µL |
| Agarose Pouder | 2gă |
| Ampicillin | 40µL |
Transformation
| Name | Sample | Competent Cells | Total | Plate |
|---|---|---|---|---|
| tetR | 3µL | 19µL | 22µL | Kan |
| RBS | 3µL | 19µL | 22µL | Amp |
| lacZα | 3µL | 19µL | 22µL | CP |
| lacI | 3µL | 19µL | 22µL | CP |
| Plac | 3µL | 19µL | 22µL | CP |
