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• iGEM 2013

Phage Purification May - June Notebook: May 13 - May 26 Experiment Listing

Overview March-April May-June July-August September-October

5.20 Titer The titer will allow us to know if our purification resulted in viable phage. Detailed procedure: 5.20 Titer 5.22 T4 Titer The T4 titer will allow us to know if our purification resulted in viable T4 phage. Detailed procedure: 5.22 T4 Titer 5.24 CsCl Gradient Phage Purification This procedure uses a CsCl gradient to purify phage at a high level of purification. Detailed procedure: 5.24 CsCl Gradient Phage Purification 5.26 PEG Purification The first step in phage purification. This will purify phage using PEG 8000. Detailed procedure: 5.26 PEG Purification