Team:SDU-Denmark/Tour31

Specifications

Rewiring of the E. coli machinery

MEP pathway The biosynthesis of isoprenoids in bacteria works through the methylerythritol phosphate pathway (MEP pathway). The pathway is initiated with a condensation reaction between D-glyceraldehyde 3-phosphate and pyruvate to produce DXP 1-deoxy-D-xylulose-5-phosphate and CO2 catalysed by Dxs. 1-deoxyxylulose-5-phosphate synthase

DXP is reductively isomerized to MEP 2-C-methyl-D-erythritol-4-phosphate by Dxr 1-deoxy-D-xylulose-5-phosphate reductoisomerase in a reversible NADPH-dependent reaction and subsequently converted to CDP-ME 4-diphosphocytidyl-2-C-methyl-D-erythritol in a CTP substrate inhibited reaction catalysed by IspD. 4-diphosphocytidyl-2-C-methyl-D-erythritol synthase