Team:NJU China/Team

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Team

NJU_China

Gong Fei To strive,to seek, to find and never to give up.
Contact:gongfei1024@126.com

Dai Yimei Hi, my name is Dai Yimei and I am a senior at Nanjing University. I am majoring in life science and plan to pursue graduate study in developmental biology. I believe that science can work wonders.
Contact: yimei1112@163.com

Weng Mingxi I love science and have great passion in life science. This is my first time to participate in iGEM. I find my fun in our project this summer. It's so great to design and synthesize biological devices to achieve a certain transformation of life to some extent as our will. And it’s always a pleasure to be with everyone in our team. What a wonderful experience!
Contact: 840515989@qq.com

siRNA screening (Failed) 27th: We cultured the 293t cells in a 12-well plate. 28th: We transfected 293t cells. 29th: We collected cells and extracted RNA from it. 30th: We did RT-qPCR with the RNA we extracted on 29th April. 1st: We did qPCR with the cDNA we got on 30th April.

May

2013

siRNA screening (Failed), examination whether siRNA are capsulated into exosomes (Success) 8th: We extracted plasmids of 3 kinds of siRNA. 9th: We cultured 293t cells in eight D10 dishes and a 12-well plate. 10th: We extracted 2 kinds of over-expression plasmids and examined the concentration of it. Then we transfect these plasmids into 293t cells respectively. 11th: We collected cells and exosomes from 8 D10 dish, and cells from 12-well plate. 12th: We extracted RNA from cells and exosomes. 13th: We did RT-PCR and qPCR.

August

2013

July

2013

June

2013