Team:Chiba/Project/oxidation

Oxidation

1.Introduction

    Two proteins called glutathione and thioredoxin which have disulfide bond (-s-s-) in oxidized states exist in E.coli(WT). They play a role of redox control in E.coli.
    Oxidative stress avtivates glutathione reductase (gor) and thioredoxin reductase (trxB). Therefore, E.coli is constantly reductive.
    On the other hand, cytosol of yeast is oxidative originally and they can have magnetism, and be attracted by magnets.
    So, we thought changing the E.coli cytosol to oxidative state like yeast leads to be attracted by magnets.
    That's why knocking out trxB and gor is neccesary to acchive magnetism.

2.Materials&Methods

2.1plasmid construct

parts

2.2細胞内の酸化状態の評価

3.Results&Discussion

3.1.細胞株

3.2.細胞内の酸化状態の評価

4.Conclusion

それは・・