Team:HokkaidoU Japan/Notebook/Promoter R0040,R0010-I732020

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Maestro E.coli

Notebook

R0040,R0010-I732020

  • Start

  • Transformation

    R0010, R0040 from distribution kit
    each of 2µL DNA to JM109

  • Culture

    R0010, R0040

  • Mini-prep

    R0010, R0040

  • Digestion

    Cut R0010, R0040 with SpeI, PstI (using 10×H buffer)
    Cut I732020 with XbaI, PstI (using 10×M buffer)

  • Antarctic phosphatase

    R0010, R0040

  • Ligation

    Ligate (R0010, R0040) with I732020

  • Transformation

    (R0010, R0040)-I732020
    each of 2µL DNA to JM109

  • Colony PCR & Culture

    (R0010, R0040)-I732020

  • Mini-prep

    (R0010, R0040)-I732020

  • Complete!

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