Team:UTK-Knoxville/primers

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Team:UK-Knoxville - 2013.igem.org

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Primers

Name Sequence Melting Temp.(°C) Function/Comment Status
General (Transmembrane)        
EnvZ_r aaaaaactcgagttaggatccTTACCCTTCTTTTGTCGTGCC 57 Lowercase part has restriction sites: XhoI-ctcgag BamHI-ggatcc In
OmpR_r AAAAAACTCGAGAGGGGATCtcatgctttagagccgtccgg 60 Restriction sites: XhoI-ctcgag BamHI-ggatcc In
OmpR_f aaaaaaCATATGCAAGAGACCTACAAGATTCTG 53 Restriction site: NdeI-catatg In
         
Aer        
Aer_f aaaaaagaattctaaagatctaaggagatataATGTCTTCTCATCCGTATGTC 53 Lowercase part has restriction sites: EcoRI-gaattc BglII-agatct In 5/30
Aer-EnvZ_1r GCGCAAGTCGTGACTTACCCatttctgacactggacacctgg 58 Overlapping PCR primers In 5/30
Aer-EnvZ_1f ccaggtgtccagtgtcagaaatGGGTAAGTCACGACTTGCGC 59 Overlapping PCR primers In 5/30
         
NarX        
NarX_F       We can order both sets of primers (transmembrane region from either protein)
NarX_R        
Nar-EnvZ_R        
         
HemAT        
HemAT_F aaaaaagaattctaaagatctaaggagatataTTGTTATTTAAAAAAGACAGAAAACAAG 52 Lowercase part has restriction sites: EcoRI-gaattc BglII-agatct Ordered 6/12
HemAT_R ggcgctggttatccatcggactggttgtactcgctttgaaacg 58 Overlapping PCR primers Ordered 6/12
HemAT_NtrB_F cgtttcaaagcgagtacaaccagtccgatggataaccagcgcc 61 Overlapping PCR primers Ordered 6/12
         
TodS        
         
         
         
         
RcoM        
RcoM_F aaaaaagaattctaaagatctaaggagatataTTAGTTTTTACTTGCTTCAGTTGG 54 Lowercase part has restriction sites: EcoRI-gaattc BglII-agatct Ordered 6/12
RcoM_R ggcgctggttatccatcggaatgtttaaatattttgcaattgcttc 52 Overlapping PCR primers Ordered 6/12
RcoM_NtrB_F gaagcaattgcaaaatatttaaacattccgatggataaccagcgcc 61 Overlapping PCR primers Ordered 6/12
         
LasR        
         
         
         
         
Cytoplasmic Construct        
NtrC_F aaaaaacatatgatgcaacgagggatagtctgg 58 Restriction site: NdeI-catatg Ordered 6/12
NtrC_R agatccgagctggggatggagtgactcgagaaaaaa 57 XhoI-ctcgag Ordered 6/12
NtrB_R aaaaaactcgagttaggatccTTATTTCCTGATAGGCAGGTAAAC 54 Lowercase part has restriction sites: XhoI-ctcgag BamHI-ggatcc Ordered 6/12
         
         
         
         
         
         
         
         
The backbone will be cut with NedI on the OmpR and NtrC side and BamHI on the EnvZ and NtrB side        

 

 

 

 


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