Team:HokkaidoU Japan/Notebook/RBS SD8-1C3-Replacement

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Maestro E.coli

Notebook

SD8-1C3 Replacement

  • Start

  • Mini-Prep

    oripro on 1C3

  • PCR

    R0040-SD8-LacZα-B0015 100up 200dn

  • Gel Extraction

    R0040-SD8-LacZα-B0015

  • Digestion

    Cut R0040-SD8-LacZα-B0015 on 1A2 and Oripro-B0034-LacZα-B0015 on 1C3 EcoRI and PstI (using 10×H buffer)

  • Gel Extraction

    Oripro-B0034-LacZα-B0015 R0040-SD8-LacZα-B0015

  • Ethanol Precipitation

    Oripro-B0034-LacZα-B0015 R0040-SD8-LacZα-B0015

  • Ligation

    Ligate R0040-SD8-LacZα-B0015 with Vector(pSB1C3)

  • Transformation

    R0040-SD8-LacZα-B0015
    each of 5µL DNA to DH5α

    faild

  • Transformation

    R0040-SD8-LacZα-B0015
    each of 5µL DNA to DH5α

  • Colony PCR

    R0040-SD8-LacZα-B0015

  • Culture

    R0040-SD8-LacZα-B0015

  • Mini-prep

    R0040-SD8-LacZα-B0015

  • Complete!

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