Team:BostonU/Protocols

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BostonU iGEM Team: Welcome


Protocols

This section contains the protocols that we used during the summer and that are mentioned in our Notebook.

We hope they help anyone interested in our experiments to successfully repeat them.


  • DNA Quantification

  • Miniprep

  • PCR Cleanup

  • Ligation

  • Media Prep

  • MoClo Level 0 Reaction

  • MoClo Level 1 and 2 Reactions

  • Phusion PCR

  • Restriction Digest

  • Restriction Digest 10 µL mapping

  • Transformation

  • Gel Extraction

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