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From 2013.igem.org

Researcher and Lab Safety

Organisms

All the organisms utilized in the Haynes Lab comply with biosafety level 1 (BSL1) and do not possess the potential to cause disease in individuals. All organisms, despite having no association with disease, are treated as potential pathogens, thus personal protective equipment such as gloves, laboratory coats, and protective eyewear/goggles are used to prevent contact with bacteria and yeast samples in the lab, to prevent splashes, and to avoid sources of ultraviolet radiation.

Standard protocols were followed for all genetic manipulation, including PCR, plasmid assembly (restriction, ligation, and transformation), and DNA extraction. These protocols standardize specific safety procedures encountered in day to day labwork.

The following organisms are present in the lab and directly utilized in our project:

• Escherichia coli BL21(DE3)^{[http://www.ncbi.nlm.nih.gov/bioproject/30681]}
• Escherichia coli NEB-10 Beta^{[http://www.neb.com/nebecomm/products/productc3020.asp]}
• Human Dendritic Cells