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Team:Chiba/Notebook
From 2013.igem.org
week1(8/18/2013~8/25/2013)
8/22//2013
.Extracting the human ferritin plasmid and iron chaperone plasmid
8/23/2013
.BBa_I746908 and BBa_I746902 transformation
8/24/2013
.Extracting BBa_I746908 and BBa_I746902
8/25/2013
week2(8/26/2013~9/1/2013)
8/26/2013
8/27/2013
8/28/2013
8/29/2013
.Gel electrophoresis(ferritin,iron chaperone4-6c,4-14I)
.PCR:(4-6c),ferritin(mid,strong)
.Digestion test:4-6c,ferritin,iron chaperone
.Pre-culture:CRISPRi
8/30/2013
.Extracting CRISPRi plasmids(fur,fieF,gor,and trxB)
.PCR:ferritin(min,strong),CRISPRi(fur,fieF,for,and trxB)
.Gel electrophresis:PCR products
.Gel extractions:PCR products
8/31/2013
.Digestion:ferritin(mid and strong),4-6c
9/1/2013
.FASTR:CRISPRi
.Ligation:ferritin and 4-6c
.Begining editing Wiki
week3(9/2/2013~9/7/2013)
9/2/2013
.Making plates with Broth and methylene blue(MB)
9/3/2013
.Making olates with Broth and MB
.Culturing BL21 and shuffle , respectively
9/4/2013
9/5/2013
9/6/2013
9/7/2013
week4(9/8/2013~9/14/2013)
9/9/2013
.Hold a presentation(state of progress)
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9/10/2013
9/11/2013
9/12/2013
9/13/2013
9/14/2013
Project
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| Iron Uptake | Iron Storage | Iron Oxidation |
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