Team:Arizona State/BSE

From 2013.igem.org

Revision as of 20:24, 27 September 2013 by Napatel (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Overview

We are utilizing the pOSIP plasmid to chromosomally integrate transformed genes into NEB 10 Beta cells and then remove antibiotic resistance genes to prevent horizontal gene transfer of LLO or any antibiotic resistance genes. We are also developing a pH-based gene switch to create a secondary safety mechanism so that LLO is only translated under acidic conditions and translation is repressed by neutral and basic conditions.