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Team:Evry/Notebook
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You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
Labwork:
Friday, June 21st
The three following E. coli strains have been prepared to be chemically competent: BL21, DH5𝝰 and TOP10. To check the quality of our work as well as their competence, each strain has been plated on LB medium with ampicillin, kanamycin or chloramphenicol. We also transformed our bacteria with a random plasmid and plated them on LB medium with ampicillin only. We incubated our petri dishes for the week end at 30°c
Monday, June 24th
Competents cells made on friday 21st was plated and incubated the whole week-end at 30°C. Today we analyzed results and it seems that medium had been contaminated.
New competents cells will be made.
Tuesday, June 25th
New competent cells have been made with the same strains of bacteria. We thought that the contamination of our cells was due either to the solution of CaCl2 that we used which may had not been sterile and/or incorrect manipulations. This time, we prepared new CaCl2 solutions that we made sure to autoclave correctly. Then, same tests as we did before of contamination and competence have been made.
Wednesday, June 26th
The DH5𝝰 strain was not contaminated and competent. The TOP10 strain was contaminated only on the Kanamycin petri dish and competent. The BL21 strain was contaminated and not usable.
Thurdsay, June 27th
We designed the plasmid constructions and primers we will use.
