Team:UCLA/Notebook/Biobrick

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• HOME
• PROJECT
• TEAM
• PARTS
• MODELING
• HUMAN PRACTICES
• NOTEBOOK
• SAFETY
• ATTRIBUTIONS
• JUDGING

• Overview
• Mtd Biobrick Construction
• mRNA Display Library Generation
• mRNA Display

Making the Mtd Biobrick

To create this biobrick, we utilized a combination of splicing overlap extension PCR and Gibson Assembly to extract a sequence from the BPP-1 phage's genomic DNA that would be free of the standard biobrick restriction enzyme sites. The protocols for those methods are listed below:

PCR to generate fragments of mtd

In this step, four separate fragments of the mtd gene are created via PCR. This step is necessary to eliminate illegal internal biobrick sites (EcoRI, PstI, NotI) and to add necessary biobrick sites to the 5' and 3' ends.