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From 2013.igem.org

• Home
• Project
  • Overview
  • Cell Polarization
  • Ion Pumps
  • Biofilm Membrane
• Notebook
  • Notebook 1: Polarization
  • Notebook 2: Pumps
  • Notebook 3: Biofilm
  • Safety
• Team
  • Team Members
• Acknowledgements

The primary goal of the Biofilm Membrane team was to produce a high-density monolayer biofilm. A monolayer biofilm structure is critical to the function of the biomachine as the overall goal of the project is to move salt across a membrane, producing potable water. Caulobacter Crescentus (C. crescentus), is a polar body bacteria that forms high density monolayer biofilms and has been well characterized to support its use in biotechnological applications. The biofilm forming capabilities of a strain of C. crescentus (CB15) have been well characerized by a group at Stanford University whose work we have cited. CB15 was obtained from the Standford lab for the purpose of this project.

In order to validate the formation of a high-density monolayer biofilm we grew CB15 on three different materials: glass, plastic, and micro-filter paper.