Team:HokkaidoU Japan/Shuffling Kit/Primer Designer

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Maestro E.coli

Optimization Kit

POK-ROK Primer Designer

1. First, you have to decide whether or not you conform to our default overhang set.

Yes, I conform to the default.

No, I design original overhangs.

2. Secondly, you hove to determine how many CDS are included in your target plasmid.

I have CDS(s) included.

3. Then, please select the region to where your flagment correspond in target plasmid.

1

2

3

4

5

6

7

8

9

Beginning of the flagment:

End of the flagment:

4. Okey, now we are ready to go. Enter your flagment's sequence, and press "Calculate"!

2. Please enter overhangs and flagment's sequence, and press "Calculate"

Forward

sequence

tm

Reverse

sequence

tm

5. Now, repeat previous step for remaining flagments included in the target plasmid.

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