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From 2013.igem.org

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Biobrick Abstract

Restriction Enzymes are necessary tools in synthetic biology, without them, biobrick assembly would be impossible. These restriction enzymes are also very pricey and prove to be a significant cost in labs everywhere. Because these enzymes are expensive, but necessary, experiments in synthetic biology are limited to companies and universities with high budgets. Here at the University of Colorado-Boulder, we aim to find a technology that lowers the costs of these restriction enzymes and open the field of synthetic biology to more people. Our solution to this problem we are trying to create is a "BetaBioBrick." Essentially we are trying to come up a with low cost method for labs to create their own restriction enzymes so they won't have to order from pricey suppliers. We attempting to do this by creating a BioBrick part that will house the genes for a restriction enzyme (EcoRI, XbaI, ApoI), their relative methylase, and if needed a method of purification.