COLONPCR Protocol

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Georgia Tech iGEM

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COLONY PCR Protocol for PCR machines

98ºC for 2 min
98ºC for 15 sec
60ºC for 15 sec
72ºC for 2 min
repeat steps 2-4 31 times
72ºC for 4 min
Hold at 4ºC

Estimated Time: 1 hr 45 min

Retrieved from "http://2013.igem.org/COLONPCR_Protocol"

Category: Protocol