Team:DTU-Denmark/Notebook/1 July 2013

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Main purpose today

Make M9 Medium for the biolector toxicity experiment

Who was in the lab

Helen, Gosia, Ariadni

Procedure

According to open wetware protocol for Biolector Experiment

Media: 1L of media

  • 10 mL Casamino Acid ( ready solution from Andreas)
  • 2 mM MgSO4 (from 2M liquid stock)
  • 0.1 mM CaCl2 (liquid solution with 15% gly)
  • 200 mL M9 salts

fill to 1 L with sterilized water

1 L stock of M9 salts:

  • 64 Na2HPO4*7H2O = 31.2 Na2HPO4
  • 15 g KH2PO4
  • 25 g NaCl
  • 5 g NH4Cl
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