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Team:Tsinghua-E/Result
From 2013.igem.org
Strains
BL21(DE3)(mut-bitter30(+))/pSC101wt-ori-mutD-sfGFP/pTrc99A-trpss-30-tetA
BL21(DE3)(mut-bitter32(+))/pSC101wt-ori-mutD-sfGFP/pTrc99A-trpss-32-tetA
Negative control：
BL21(DE3) pSC101wt-ori-mutD-sfGFP/pTrc99A-trpss-30-tetA
BL21(DE3) pSC101wt-ori-mutD-sfGFP/pTrc99A-trpss-32-tetA
Each evolution was conducted in three parallel with only one parallel of negative control.
Results
By using the parts constructed by us, following the protocol described, we conducted the continuous evolution derived by mutD based mutation for two E. Coli BL21 (DE3) strains carrying bitter defender part 30 and 32. The results were shown below:
Table1 Titer increase dynamics of different strains after different rounds of evolution (rbs30)
|
1st generation |
2nd generation |
3rd generation |
Control (only have mutation part) |
No growth |
No growth |
No growth |
Bitter defender part rbs30 (mg/L) |
0.791 |
3.578 |
3.119 |
Bitter defender part rbs30/mutation part (mg/L) |
0.500 |
0.919 |
2.245 |
Table2 Titer increase dynamics of different strains after different rounds of evolution (rbs32)
|
1st generation |
2nd generation |
3rd generation |
Control (only have mutation part) |
No growth |
No growth |
No growth |
Bitter defender part rbs32 (mg/L) |
0.673 |
1.063 |
3.433 |
Bitter defender part rbs32/mutation part (mg/L) |
3.142 |
0.766 |
2.540 |
