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Team:Nevada/Notebook/Month4
From 2013.igem.org
August 5th – August 11th
Monday
Christian and Jasmine started Top 10 Cultures for first assay.
Jon transformed LMG194 with pBAD vector containing INPN-YFP
Tuesday
Christian and Jasmine did the first lysozyme only assay.
Wednesday
Becca, Christian, and Jasmine started a culture for TOP10 cells to be used in second assay. They also purified Lyz103 and KZ144 endolysin.
Jon induced LMG194 and BL21 cells containing cell surface fluorescent markers
Thursday
Christian and Jasmine ran the Xanthomonas endolysin assay using 30 ul of purified endolysin.
Jon noted fluorescence in BL21 cells but not LMG194
Friday
Christian and Jasmine checked assay 2 results and plated contents from E11, E12, F11, and F12.
Christian, Jasmine, and Dafne completed project description.
Jon began growing large culture of Plac-INPN-YFP containing BL21 cells for assay
August 12th – August 18th
Monday
Christian grew RFP containing bacteria cells to be used in an assay.
Tuesday
Christian and Jasmine Worked on The RFP assay.
Wednesday
Christian and Jasmine observed the data for assay.
Thursday
Everyone prepared for poker night
Friday
The team had the Poker Night fundraiser
August 19th – August 25th
Monday
Christian did a transformation n for Cody’s submission plasmid. He also started a small culture of TOP 10 cells for assay.
Tuesday
Christian performed the assay on the top 10 cells and used protease inhibitor this time. Christian also did a transformation for Jon’s part.
Christian cleaned and organized the lab.
Wednesday
Christian dissolved pBAD and Xanthamonus endolysin primers. He also examined assay results and cleaned the lab.
Thursday
Jon cleaned lab
Friday
Christian grew cells for assay
Saturday
Christian performed assay.
Sunday
August 26th – September 1st
Monday
First day of school for all.
Christian, Jon, and Jasmine started organizing the citations for the papers used in this project. They also worked on the abstract.
Tuesday
Christian, Jon, and Jasmine worked on abstract and paper citations.
Wednesday
Christian started a small colony for the assay.
Thursday
Christian and Jasmine did another assay with lysozyme with Pseudomonas aeruginosa cells
Friday
Christian cleaned labs, threw away old buffers, and washed dishes.
Saturday
Christian and Jasmine worked on assay.
September 2nd – 8th
Monday
Christian grew cells for assay
Tuesday
Christian did the assay.
Wednesday
Thursday
Becca, Christian, and Jasmine did a lysozyme assay (but in eppendorf tubes) with Pseudomonas aeruginosa and got successful results.
Friday
Becca, Christian, Dafne, Jon, and Jasmine discussed our project and financial situation with the provost.
Saturday
Sunday
September 9th – 15
Monday
Tuesday
Christian, Behdad, Jasmine, and Jon worked on finishing the submission plasmid portion of the project.
Wednesday
Christian, Behdad, Jasmine, and Jon worked on finishing the submission plasmid portion of the project.
Thursday
Christian, Behdad, Jasmine, and Jon worked on finishing the submission plasmid portion of the project.
Friday
Christian, Behdad, Jasmine, and Jon worked on finishing the submission plasmid portion of the project.
Saturday
The team participated in the college FIELD DAY. Dafne ate dippin dots
Sunday
September 16th – 22
Monday
Christian, Behdad, Jasmine, and Jon worked on finishing the submission plasmid portion of the project.
Tuesday
Christian, Behdad, Jasmine, and Jon worked on finishing the submission plasmid portion of the project.
September 23rd -29th
Monday
Behdad, Christian, Dafnne, jasmine, and Jon worked on the website content.
Tuesday
Behdad, Christian, Dafnne, jasmine, and Jon worked on the website content.
Wednesday
Behdad, Christian, Dafnne, jasmine, and Jon worked on the website content.
Thursday
Behdad, Christian, Dafnne, jasmine, and Jon worked on the website content.
Friday
Behdad, Christian, Dafnne, jasmine, and Jon worked on the website content.
