Team:MIT/Attributions

From 2013.igem.org

Revision as of 03:55, 28 September 2013 by Klathem (Talk | contribs)

iGEM 2012

Overview

  • Project Overview

miRNA Signal

  • Overview
  • siRNA Characterization
  • Exosome Isolation and Co-Culturing
  • Cell-Cell Co-Culturing

Protein Signals

  • Overview
  • GFP
  • rtTA3
  • Cre
  • L7Ae
  • Cas9-VP16

Novel DNA Sensor: Cas9 Split Venus Fusion

  • Overview
  • Leucine Zipper Fusion
  • DNA Sensing

Our BioBricks

  • Favorites
  • All BioBricks

Attributions

  • Attributions

Attributions

Our instructors and other individuals from outside the Weiss lab provided the training necessary for us to conduct our work. Deepak Mishra and Brian Teague, two of our advisors, taught cloning and experimental techniques, and conducted training on fluorescence microscopy on the Zeiss scope. Team members conducted Zeiss microscopy and prepared samples for FACS and confocal microscopy. An advisor operated the cytometer the confocal microscope on these samples as directed. Nathan Kipniss, a member of the 2012 MIT iGEM team, assisted with the initial tissue culture training. We were lucky to have Samira Kiani, whose work inspired the Cas9-CRISPR parts of our project, to advise our team. Her help with training and with design and assembly strategies was invaluable. We would also like to thank the 2012 MIT iGEM team for providing our wiki design. Without all of these contributions, our work this summer would not have been possible.

Otherwise, all of the data presented on the results page was generated by members of the 2013 MIT iGEM team, with the exception of FACS and some microscopy, time-lapse and z-stack images in particular.